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KMID : 0358420120550040244
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Korean Journal of Obstetrics and Gynecology
2012 Volume.55 No. 4 p.244 ~ p.249
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Intra-peritoneal NF-κB decoy oligodeoxynucleotide decreases postoperative intra-abdominal adhesion
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Ahn Byul-Nim
Song Mi-Hyun
Kim Ju-Hyun
Kim Kyung-Hyun
Park Kwan-Kyu
Choi Youn-Seok
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Abstract
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Objective: The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor κB (NF-κB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion.
Methods: Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-κB decoy oligodeoxynucleotide (NF-κB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3).
Results: Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically significant (P <0.001).
Conclusions: Intra-peritoneal NF-κB decoy ODN reduced the severity of postoperative intra-abdominal adhesion.
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KEYWORD
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Adhesion, Inflammation, NF-kappa B, NF-kappa B decoy oligodeoxynucleotide
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